Cloning can sometimes be tedious and time-consuming depending on your project needs. Our molecular biology experts can handle a wide range of cloning and expression services. Custom Cloning services are customized to meet specific needs. Your gene of interest can be obtained by PCR, RT-PCR or restriction digestion and cloned into any vector of your choice. The PCR product will be cloned directly into a T/A cloning vector or digested with restriction enzymes and sub cloned into the plasmid vector of your choice All PCR products will be sequenced ( both DNA strands) to ensure sequence integrity.
Mutagenesis technology is the most popular method used to modify or optimize genes, which is also a powerful tool to study gene sequences and analyse the relationship between protein structures and functions
• High Quality cDNA Synthesis; First strand cDNA : 0.1 micrograms of RNA. || Second strand cDNA
• Site Directed Mutagenesis
• Screened DNA Sequences
• Plasmid Preparation
• RACE Cloning Service
• PCR Cloning & Sub Cloning
• Cloning of synthetic gene
OUR ADVANTAGE :
We undertake the whole project for cloning services . We follow the standard protocols for different arrays for analysis :
• Reliable verification of clones by sequencing
• High throughput and multiple fragment cloning
• In-house construction and Plasmid purification
• Template DNA or RNA, or their description
• Sequence and description of the DNA to be cloned.
• Vector description
• Primer design for PCR or RT-PCR
• Cloning into the vector of your choice.
• Sequencing of target DNA in both directions.
VERIFICATION OF CLONING :
We provide high quality cloning service at very competitive price. All clones are sequenced verified and guaranteed error-free.
TECHNOLOGIES AND FACILITIES :
Microbiology Lab, Protein Chemistry Lab with Biorad FPLC, Ion Exchange Chromatography,
Preparative HPLC, 2-D Electrophoresis, Blotting Apparatus, GC-MS-MS, Triple Quadrupole, Gas
Chromatograph ( FID, FPD and NPD Detectors ), Analysis by LC-MS-MS, Analysis by HPLC - PDA,
FLD Detectors, TLC, HPTLC, UV-Visible spectrophotometer, ELISA, PCR & Real Time PCR,
Sequencing, Ion Meters, RI Detector, Flash Point Apparatus, Culture Facility etc.
Kindly mail at : firstname.lastname@example.org or Whatsapp : 9891179928