Professional Amino Acid Analysis (AAA)

HPLC & Ion-Exchange Chromatography

Accurate amino acid analysis (AAA) is a critical tool across multiple scientific sectors, including recombinant protein characterization, nutritional assessment of food products, and animal feed quality control. Whether amino acids exist in a free state or are bound in complex linear chains within peptides and proteins, precise quantification is essential.

- Complete Amino Acid Profile : A full breakdown of all protein-bound and free amino acids.
- Amino Acid Profile (Liquid Hydrolysis ): Standardized acid hydrolysis for protein characterization.
- Profile Without Tryptophan : A cost-effective solution for acid-stable amino acid determination.
- Specialized Sulfur & Essential Profile s: Comprehensive analysis including                       Hydroxyproline,Taurine, Cysteine,Methionine, and Lysine (+9).
- Standard Protein Hydrolysate : Evaluation of the constituent amino acids following protein        breakdown.

Our laboratory quantifies the full spectrum of natural amino acids, including:

Acidic : Aspartic acid, Glutamic acid, Asparagine, Glutamine.
Basic : Lysine, Histidine, Arginine.
Aliphatic/Non-polar : Alanine, Glycine, Valine, Leucine, Isoleucine, Proline.
Sulfur-containing : Cysteine, Cystine, Methionine.
Aromatic/Hydroxyl : Threonine, Serine, Tyrosine, Phenylalanine, Tryptophan ..

We provide detailed information regarding the relative amino acid composition and free amino acids. The amino acid analysis procedure includes hydrolysis, separation, detection, and quantification.

Ion-Exchange Chromatography & Ninhydrin Detection : We utilize the industry-standard method of Ion-Exchange Chromatography (IEC) paired with post-column Ninhydrin derivatization. This method is preferred for its exceptional qualitative and quantitative precision. .

Analysis of amino acids is performed with HPLC UV & Fluorescence detector and FPLC/LPLC System by using classical ion-exchange liquid chromatography with post-column Ninhydrin derivatization and photometric detection.

Why Choose Our Amino Acid Testing?

From determining relative amino acid composition to identifying free amino acids in biological samples, our services provide the granular data needed for nutritional labeling, pharmaceutical purity testing, and biochemical research.

 

CONTACT :  
Email: : info@allelelifesciences.com  or Whatsapp  +91- 8377082003