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Antibody Purification

 Antibody Purification

We offer a variety of methods for antibody purification from serum, ascites, and culture supernatants. We use a variety of methods for the purification of antibodies from serum, ascites, and culture supernatants, including ammonium salt precipitation, ion exchange and size exclusion chromatography, IMAC, GST, protein A, and protein G affinity chromatography, and custom affinity chromatography.

Services We Offer :

Affinity purification permits isolation of antibodies against the immunizing antigen, thereby eliminating cross-reactivity that would be present in unpurified serum. Antibodies of extremely high purity and specificity can be obtained by using custom affinity chromatography.
Protein A, G, and A/G Purification
High yields of pure IgG can be obtained in a single-step purification using Protein A affinity chromatography, which differ in their ability to bind antibodies
Ammonium Sulphate Precipitation
Because of their higher hydrophobicity, antibodies precipitate at lower concentrations of ammonium sulphate than most proteins and other serum components. Ammonium sulphate precipitation is then frequently used to enrich and concentrate antibodies from serum, ascitic fluid or cell culture supernatant.

Size Exclusion Chromatography
Dialysis membranes, size exclusion resins, and diafiltration devices that feature average molecular weight cut-offs can be used to separate immunoglobulins from small proteins and peptides.

Ion Exchange Chromatography
Ion exchange chromatography uses positively or negatively charged resins to bind proteins based on their net surface charge in a buffer with a given pH, according to their isoelectric point (pI)

Recombinant Protein Purification
Recombinant proteins or tagged proteins can be purified by affinity chromatography using charged or immobilized resins.

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