Contract Research Lab|Research Product|Product Testing

Antibodies, vaccines, and nucleic acid products are examples of bioformulations, which comprise the majority of therapeutic drugs available today. Research in genetics and protein biology are among the phases these bioformulations go through. Proteins can now be manufactured for use in medicinal applications. Because of this, a significant portion of the drugs being developed today are proteins produced by biotechnology. Proteins are challenging for pharmaceutical scientists to isolate, purify, formulate, and transport.

1. Pharmaceutical Biotechnology
   
   Unit I- Protein Extraction and Analysis
   Protein Extraction from Cultured Cell
   Quantification of Protein - Bradford / lawry's Method
   Analysis of Protein by SDS-PAGE
   Densitometric analysis of proteins

2.  Unit II- Partial Purification and Dialysis
   Acid Base Equilibrium, pH, Buffer System, Charge, pI and pKa Value and Reagent Preparation
   Ammonium sulfate precipitation Assay
   Estimation of Protein after Ammonium sulfate precipitation
   Dialysis or Desalting of Protein Sample

3. Unit III-Protein Purification by Fast Protein Liquid Chromatography
   Preparation for Protein Purification Strategies
   Purification of Protein by Size Exclusion Chromatography
   Affinity Chromatography Purification
   Protein Purification by Ion Exchange Chromatography

4. Unit IV-Protein and Amino Acid Analysis
   Analysis of Protein by SDS-PAGE & Western Blot
   Western Blot Data Analysis
   Separation & Detection of Amino Acids by HPLC
   Amino Acid Quantification Data Analysis

5. Frequently Asked Questions
   

6. What is a Biopharmaceutical Biotechnology Lab?

   Fume Hood: In genetic and molecular biology labs, PCR hoods are used to stop sample contamination.

   Fast Protein Liquid Chromatography: Protein liquid chromatography (FPLC) usually uses a resin-crosslinked agarose bead as the stationary phase and a buffer as the mobile phase. Using size exclusion, affinity, and ion exchange chromatography, protein purification can be standardized and performed using the automated FPLC system.

   Preparative HPLC: The primary characteristic of RT-PCR is the "real time" detection of DNA amplification while the PCR is running. Using a "fluorescent reporter," which monitors the fluorescent signal to determine the amount of amplified DNA molecules directly, the PCR progression is evaluated.

   Microplate Reader: Thousands of DNA sequences can be analyzed simultaneously using microarrays for genetic research and diagnostic purposes.

   Analytical HPLC: The DNA Concentrator is used to efficiently concentrate DNA/RNA from various samples.

   Analytical HPLC: The DNA Concentrator is used to efficiently concentrate DNA/RNA from various samples.

   Other Basic Lab Equipments:  Other equipments used in molecular biology labs are distilled water unit,ICE flaking machine,electrophoresis units, Gel documentation system with gel analysis software, linear current power supply, refrigerated centrifuges, water bath,dry bath etc.

   General Lab Techniques

   Bioinformatics: There are several great online tools and platforms for learning bioinformatics, depending on your level and interests like; IIT virtual Learning Platform,NCBI Bioinformatics Resources.

   Basic Lab Techniques: Basic calculation of chemical preparation (use graph pad or molarity dilution app), buffer preparation, Pipetting,pH, sterlization etc is included in every course..

   Note: All basic experiments of qualitative and quantitative analysis of various types of products, basic microbiology, basic in vitro cell culture, and PCR-based experiments will be available on our YouTube channel from time to time.

   Our Pedagogy

   Our Pedagogy evolve to make education more experiential, inquiry-driven, discovery-oriented, learner-centred, discussion-based, flexible, and enjoyable.

   Validated Lab Manual provides a platform for understanding the basic of experiments

   Be a volunteer oppurtunity- Assisting fellow lab students will boost your self-esteem and cultivate your leadership skills.

   Our Guidance- Lab scientists and technicians will have one-on-one interactions with students and help them develop their own lab abilities.

   Eligibility,Fee and Duration

   Eligibility: Students from life science, biotechnology, chmeistry, forensic science,food science and pharmaceutical science may apply.

   Fee: INR 12,000/- For Indian Citizens.

   Duration: 85 Hours or Upto One Month (maximum)

   Important information

   State of art lab facility: We provide the best lab space for working in chemistry and biology. Students use cutting-edge tools firsthand while being supervised by knowledgeable team members.CHECK LAB FACILITY

   Bridging the Gap Between Theory and Practice:

   Immersive Learning: Students who immerse themselves in practical activities get essential insights and experience that help them prepare for issues they may face in their future employment.

   Application of In-vitro Cell Culture: Students who immerse themselves in practical activities get essential insights and experience that help them prepare for issues they may face in their future employment.

   How to select training place andtraining program? You are strictly advice to discuss the Full Summer Training Brochure with your Faculty of your institution or Scientist in your relation.Their advice will be crucial for your career.

   Employability & Application of Module: Biopharmaceutical Industry,Pharmaceutical Industry, Enzyme Production, Food Industry, Vaccine Research, Cancer Research, Cosmetics and Consumer Products Analysis, Agricultural and Environmental Sciences.

   

   CONTACT : 
   Email:: info@allelelifesciences.com  or Whatsapp +91- 8377082003