Highly sensitive biochemical methods called Fluorescenceassays are employed to determine whether a certain molecule is present in a sample or how much of it is there. Fluorescence, or the emission of light by a material that has absorbed light or other electromagnetic radiation, provides the basis for these assays. .

- FluorescenceEnzyme Assays
- Quantitation of Biomolecules
- Cell Viability and Proliferation
- Fluoroscence Binding Studies

FluorescenceEnzyme Assays

Fluorometric enzyme assays are typically far more sensitive than spectrophotometric assays, they may be hampered by impurity interference and the volatility of many fluorescent substances in the presence of light..

Quantitation of Biomolecules

Quantitation of biomolecules using a fluorometer involves measuring Fluorescence intensity from a fluorescent probe or dye that binds specifically to the target molecule. This technique is highly sensitive and suitable for quantifying DNA, RNA, proteins, or small molecules in various samples..

Cell Viability and Proliferation

Using a fluorometer, assays for cell viability and proliferation entail identifying Fluorescence signals that distinguish between living, dead, and proliferating cells. These tests use fluorescent dyes or probes that react to particular biological functions including DNA synthesis, metabolic activity, or membrane integrity...

Fluoroscence Binding Studies

Fluorescence binding studies are a powerful tool for analyzing the interactions between molecules, such as ligand-receptor, protein-protein, or protein-DNA interactions.

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