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Methylation Analysis Services

Methylation analysis Services :

Methylation analysis is the study of the chromosomal patterns of DNA or histone modification by methyl groups. This type of modification affects CG dinucleotides and involves the covalent addition of a methyl group (CH3). Lysine residues in histone tails and the cytosine (C) base in DNA can both be methylated.

- COMET Assay
- Methylation Analysis by PCR
- Me
thylation Analysis by Sequencing
Analysis by HPLC  by estimation of 5-mC or 5-hmC
- Me
thylation Analysis by ELISA ( measurement of 5-mC or 5-hmC )

COMET Assay:
DNA strand breakage assay also known as single cell gel electrophoresis assay is a sensitive and rapid test for quantifying and analysing DNA damage in individual cells.

DNA Metylation Analysis by Sequencing :
One of the most commonly used techniques for detecting DNA methylation at specific genes or sequences is methylation-specific PCR. The methylated regions are then selectively amplified using primers designed for methylation and sequenced after the DNA's cytosine is bisulfite-converted to uracil.

In Whole Genome Bisulfide Sequencing -
Bisulfite conversion PCR primer design PCR amplification optimization Library preparation QC of final libraries (DNA concentration, analysis of the profile) Sequencing on Illumina platform

Bisulfide Modification and Methylation HPLC Analysis :
We offer high-performance liquid chromatography analysis services that are effective and precise. Exploring the methylation states of genomic DNA is possible after bisulfite treatment. Cytosine that has not been methylated is transformed to uracil and finally to thymine. It is possible to measure the concentration of 5 mC (5-methylcytosine) on DNA molecules and PCR products by sequencing and mapping to a sufficient read depth.

Methylation Analysis by ELISA

The genomic DNA is denatured and fixed, and the methylated cytosines are detected through sequential incubation steps with: (a) a primary antibody raised against 5 mC; (b) a labeled secondary antibody; (c) colorimetric or fluorometric detection reagents; and (d) measurement in a microplate reader. ELISA is a reliable method that can be used to detect epigenetic modifications of DNA immobilised on plastics.

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