Protein Extraction Services

We listen to your requirements, understand your purposes, discuss with you about the possibilities, design and optimize the strategies, and walk you through your research projects.
Your custom protein expression will be optimized by engineering an expression construct with a codon-optimized DNA insert, a strong promoter, an efficient ribosome binding site, a high copy number and combining it with an appropriate host.

Our Services :

Insilico Designing of Gene of Interest & Synthesis / Generation of Gene by PCR
Subcloning into an appropriate vector
Confirmation of DNA Insert by sequencing
Transformation in an appropriate host
Optimisation and Production of Protein
Purification, Quantification and Confirmation by ELISA, SDS-PAGE & Western Blot

Your custom protein expression will be optimized by engineering an expression construct with a codon-optimized DNA insert, a strong promoter, an efficient ribosome binding site, a high copy number and combining it with an appropriate host.

Gel Filtration
Ion Exchange
Affinity Chromatography
Hydrophobic Interaction

Facility Available for Protein  Extraction :

• PCR & Real Time PCR
• Cloning & Expression Facilities
• Sequencing Facility
• Akta Prime FPLC in temperature controlled environment
• Biorad Profinia For Affinity Purification
• Preparative HPLC
• Agilent HPLC

CONTACT : 
Kindly mail at : allelelifesciences@gmail.com or Whatsapp +91 - 9891179928 | Direct Contact : 8377082003