Protein Extraction Services:Allele Life Sciences

Protein Expression & Extraction:

We listen to your requirements, understand your purposes, discuss with you about the possibilities, design and optimize the strategies, and walk you through your research projects.
Your custom protein expression will be optimized by engineering an expression construct with a codon-optimized DNA insert, a strong promoter, an efficient ribosome binding site, a high copy number and combining it with an appropriate host.

Services We Offer :

Steps	Process	Details
Step - 1	IInsilico Designing	Bioinformatics analysis of Gene of Interest
Step - 2	Codon Optimisation	Selection of Plasmid, Vector and Gene Synthesis
Step - 3	Cloning & Sub cloning	Sub Cloning into suitable expression vector
Step - 4	Cell Transformation	Transformation into an appropriate host
Step - 5	Screening Studies	Screening for the best expressing protein variant
Step - 6	Protein Production	Expression Evaluation & Optimization of protein
Step - 7	Quality Control Analysis	SDS - PAGE Analysis & Q.C Report
Step - 8	Protein Characterisation	Analysis by ELISA, Western Blot ( Client Specification)

- All clones and sub-clones generated
- All-inclusive from cloning to purification
- Sequence data of the final clone
- Guaranteed yield: 3 mg purified protein
- Guaranteed purity: > 75 % protein purity
- Quality assurance certificate

AVAILABLE FOR ADDITIONAL COST :
• Sequence Verification by Mass Spectrometry
• Endotoxin Quantification
• Endotoxin Removal
OUR ADVANTAGE -
- State-of-the-art lab with sophisticated equipment facility
- Competent, Qualified and Experienced analysts
- Fast turn-around time
- Results you can rely on

TECHNOLOGIES AND FACILITIES :
Microbiology Lab, Protein Chemistry Lab with Biorad FPLC, Ion Exchange Chromatography, Preparative HPLC, 2-D Electrophoresis, Blotting Apparatus, GC-MS-MS, Triple Quadrupole, Gas Chromatograph ( FID, FPD and NPD Detectors ), Analysis by LC-MS-MS, Analysis by HPLC - PDA, FLD Detectors, TLC, HPTLC, UV-Visible spectrophotometer, ELISA, PCR & Real Time PCR, Sequencing, Ion Meters, RI Detector, Flash Point Apparatus, Culture Facility etc.

CONTACT:
Kindly mail at : allelelifesciences@gmail.com or WhatsApp : +91- 9891179928 | Direct Contact: 8377082003

Protein Extraction Services

Protein Expression & Extraction:

Services We Offer :

We understand your laboratory testing and scientific research needs and are here to help you ..