Real-time PCR (Polymerase Chain Reaction) services are laboratory services that simultaneously amplify and quantify a specific DNA or RNA molecule in real time using a molecular biology technology. Numerous applications, such as pathogen detection, genetic research, and illness diagnostics, depend on these services..
- Pathogen Detection and Diagnosis
- Gene Expression Analysis
- Genetic Testing and Diagnostics
- Quantification of DNA and RNA
- Genotyping and SNP Analysis
Pathogen Detection and Diagnosis
Real-Time PCR is widely used for the detection and quantification of infectious agents like bacteria, viruses, and fungi.This service is also used to detect pathogens in food, water, and environmental samples, providing rapid and accurate results..
- Microbial Analysis
- Genetic Resistance Testing
- Environmental Monitoring.
Gene Expression Analysis
A specific DNA segment—usually complementary DNA, or cDNA, produced from mRNA—is amplified in real-time PCR, which tracks the amplified product's buildup. Gene expression levels are inferred using the PCR product, which is proportional to the original amount of template DNA (or cDNA) in the sample.
- Reverse Transcription (RT)
- Quantification of Amplified Product
- Genetic and Developmental Studies
- Quantification of Microbial Gene Expression.
Genetic Testing and Diagnostics
To determine a person's genetic composition, detect possible genetic problems, diagnose illnesses, and forecast disease risks, medical professionals rely heavily on genetic testing and diagnostics.
- Cancer Diagnostics
- Infectious Disease Detection
- Detection of Genetic Variants and Mutations
- Quantification of Gene Expression.
Genotyping and SNP Analysis
Since real-time PCR can evaluate numerous samples at once and is fast and sensitive, it is especially well-suited for genotyping and SNP analysis. Here are some applications of real-time PCR in SNP analysis and genotyping.
