Rapid and reliable real time PCR services for gene expression

Quantitative PCR (qPCR), another name for real-time polymerase chain reaction (PCR), is a popular molecular biology method that enables real-time DNA amplification and measurement. Because it offers information on the amplification process as it occurs, real-time PCR is superior to classical PCR.

Experimental plan of real-time PCR services:

• Design consultation to understand your qPCR application
• Choice of the desired chemistry (SYBR™ Green or TaqMan)
• RNA extraction, quantitation and quality assessment from samples
• First strand cDNA synthesis
• Primer design and validation
• Analysis of amplification efficiency for each assay using standard curves
• Data analysis and full professional report with raw data

Our qRT-PCR data analysis software is used for data analysis, and data can be sent via email. The study report contains the raw Ct values, primer sequences, and the analyzed data file with complete fold-change information.

At Allele Lifesciences, we use ABI and Bio-Rad real-time PCR platforms. Following are the various services that Allele Lifesciences can provide using real-time PCR:

Quantitative Real-time PCR (qPCR) is the gold standard for the precise nucleic acids quantification in genomics research, biologics testing and drug discovery.