Biopharmaceutics industrial biotechnology course is the study of the physicochemical properties of the drug and the drug product, in vitro, on the bioavailability of the drug.The in vitro test serves as a tool to distinguish between acceptable and unacceptable drug products.The overall goal of this training area is to provide students with a visionary perspective on the drug discovery and development process..

1. Biopharmaceuticals & In-vitro Drug Analysis
   
   Unit I- Drug Selection, Basics of cell culture and Cell Isolation
   - Lab induction:- Saftey protocols,procedures,use of lab equipments and labwares
   - Selection of drug or drug in combination  and basic lab experiments
   - Preparation of Media and Reagents for Mammalian Cell Culture
   - Isolating Cells and Growing Them in Culture Media
   -Check for the cell morphology
   
   Unit II- Cell Proliferation, viability, Migration, Invasion & Cytotoxicity Assay
   - Determine of cell numbers, growth, and proliferation
   - Cell Viability Assay
   - Cell Migration and Invasion Assay
   - Cell Cytotoxicity Assay
   
   Unit III-Cell based Assay For drug screening & Flow-cytometry Analysis
   - LDH- Release Cytotoxicity Assay
   - Basics of Flow-cytometry & Software handling
   - Apoptosis Assay by Flow Cytometry Using Annexin V Staining
   - Flow Cytometry Data Analysis
   
   Unit IV-Cytogenetics Assay by Fluorescence Microscopy
   - Cell Staining Assay
   - Immunofluorescence Characterization
   - DNA Damage COMET Assay

2. Frequently Asked Questions
   

3. What is a cell culture lab?

   Dedicated Cell Culture Room:This is an essential part of a successful cell culture project; in order to prevent contamination, a separate room or space should be set aside for it.

   Biosafety cabinet: are essential for preserving aseptic conditions and preventing the spread of contaminants in cell culture labs

   Humid CO2 incubator:.To provide a physiological environment for cellular growth.

   Inverted light microscope: coupled with epi-fluorescence and CCD-cooled camera with cell analysis software are crucial imaging tools that are required for live cell imaging, cell culture applications, and other studies.

   Fluorescence Microscope:-Fluorescence serves as a detection mechanism in a fluorescent microscope. Detailed images of the characteristics of cells and tissues can be obtained using fluorescent microscopes.

   Flow Cytometry:-.Assays for cell proliferation, cell cycle analysis, apoptosis, and transfection effectiveness within cells are all included in this widely used equipment.

   Fridge, freezers (−20°C, −80°C), liquid nitrogen storage:  to store cells, cell material, and culture components.

   Other Basic Lab Equipments:  Cell culture labs require separate equipment like filtration assembly with vacuum pump, pH meter, pipettes and pipettors, centrifuges, shaker, autoclave, and water bath to avoid contamination.

   General Lab Techniques

   Bioinformatics: There are several great online tools and platforms for learning bioinformatics, depending on your level and interests like; IIT virtual Learning Platform,NCBI Bioinformatics Resources,Rosalind, EMBL-EBI, Galaxy Project, Bioconductor, You may learn drug designing by NPTEL online.(Renowned IIT Chennai experts make drug design very simple to understand through their instruction on the NPTEL platform.)

   Basic Lab Techniques: Basic calculation of chemical preparation (use graph pad or molarity dilution app), Pipetting,pH, sterlization etc is included in every course..

   Note: All basic experiments of qualitative and quantitative analysis of various types of products, basic microbiology, basic in vitro cell culture, and PCR-based experiments will be available on our YouTube channel from time to time.

   Our Pedagogy

   Our Pedagogy evolve to make education more experiential, inquiry-driven, discovery-oriented, learner-centred, discussion-based, flexible, and enjoyable.

   Validated Lab Manual provides a platform for understanding the basic of experiments

   Be a volunteer oppurtunity- Assisting fellow lab students will boost your self-esteem and cultivate your leadership skills.

   Our Guidance- Lab scientists and technicians will have one-on-one interactions with students and help them develop their own lab abilities.

   Eligibility,Fee and Duration

   Eligibility: Students from life science, biotechnology, chmeistry, forensic science,food science and pharmaceutical science may apply.

   Fee: INR 12,000/- For Indian Citizens.

   Duration: 85 Hours or Upto One Month (maximum)

   General Lab Techniques & other information

   State of art lab facility: We provide the best lab space for working in chemistry and biology. Students use cutting-edge tools firsthand while being supervised by knowledgeable team members.CHECK LAB FACILITY

   Bridging the Gap Between Theory and Practice:

   Immersive Learning: Students who immerse themselves in practical activities get essential insights and experience that help them prepare for issues they may face in their future employment.

   Application of In-vitro Cell Culture: Students who immerse themselves in practical activities get essential insights and experience that help them prepare for issues they may face in their future employment.

   How to select training place andtraining program? You are strictly advice to discuss the Full Summer Training Brochure with your Faculty of your institution or Scientist in your relation.Their advice will be crucial for your career.

   

   CONTACT : 
   Email:: info@allelelifesciences.com  or Whatsapp +91- 8377082003