Amino Acid Analysis
Amino acids are found either in the free state or as linear chains in peptides and proteins. There are 20 commonly occurring amino acids in proteins amino acids analysis is required in several areas of research, and it is a fundamental tool in product analysis. Amino acid analysis is an important tool for many scientific areas ranging from the characterisation of recombinant proteins to the nutritional assessment of food proteins and animal feed. Amino acids exist either individually as their free form, or bound in peptides and proteins. Accurate and quantitative determination of the amino acid composition and total protein content of a sample is achieved by amino acid analysis.
foodstuffs For amino Acid Analysis :
|Dairy and milk products||Feedstuff, fodder and silage||Foodstuff and pet food|
|Waste products||Raw materials||Fruit juices & soft drinks|
|Physiological, metabolic samples ||Growth media and supplements||Protein and peptide|
|Synthetic peptides||Beer & Beverages||Food Products|
Amino Acid Analysis Services :
The determination of Amino Acids using ionexchange chromatography with post-column Food Content Analysis
- Complete amino acid profile
- Amino Acid profile ( liquid hydrolysis)
- Amino acid profile without tryptophan
- Amino Acid profile with Hydroxyproline, Taurine Cysteine, methionine and lysine +9
- Standard protein hydrolysate
- Free supplemental amino acids
- Complete supplemental amino acid profile
- Available lysine and complete amino acid profile
- Glutamine (peptides and protein)
- Physiological Analysis of Biological Fluids
Analysis of common natural amino acids :
Aspartic acid , Threonine, Asparagine, Serine, Glutamic acid, Glutamine, Cysteine, Proline, Glycine, Alanine, Valine, Cystine, Methionine,Isoleucine, Leucine, Tyrosine, Phenylalanine, Phenylalanine, Lysine, Histidine, Tryptophan, Arginine
We provide detailed information regarding the relative amino acid composition and free amino acids. The Amino Acid Analysis procedure includes hydrolysis, separation, detection and quantification
The determination of Amino Acids using ion exchange chromatography with post-column ninhydrin detection is one of the most common methods for qualitative and quantitative amino acids analysis.
Analysis of amino acids is performed with HPLC UV & Fluorescence detector, Biorad FPLC / HPLC System by using classical ion-exchange liquid chromatography with post-column Ninhydrin derivatisation and photometric detection.
TECHNOLOGIES AND FACILITIES:
Microbiology Lab, Protein Chemistry Lab with Biorad FPLC, Ion Exchange Chromatography, Preparative HPLC, 2-D Electrophoresis, Blotting Apparatus, GC-MS-MS, Triple Quadrupole, Gas Chromatograph ( FID, FPD and NPD Detectors ), Analysis by LC-MS-MS, Analysis by HPLC - PDA, FLD Detectors, TLC, HPTLC, UV-Visible spectrophotometer, ELISA, PCR & Real Time PCR, Sequencing, Ion Meters, RI Detector, Flash Point Apparatus, Culture Facility etc.
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