Services

Protein Expression & Purification

We offer a number of Protein Expression Services designed to meet specific goals, ranging from expression of  full-length recombinant proteins from full-length cDNAs, EST sequence information and genomic sequences.
Protein expression and purification service package include sub-cloning into appropriate expression vector, sequence confirmation by gene sequencing, plasmid maxi preparation, optimization of protein expression, expression confirmation by SDS-PAGE and Western blotting, and purification of expressed protein to >90% purity.

OUR SERVICES :

Protein Expression Sertvices:

» Codon optimization for bacterial expression and gene synthesis of DNA insert
» Generation of bacterial expression construct(s) in specified vector
» Transformation into an appropriate host and preparation of glycerol stocks
» Screening studies for the best-expressing protein variant
» Optimization of growth conditions
» Expression assessment by SDS-PAGE/Coomassie or Western blot

Protein Purification Sertvices:

»
Affinity Chromatography ( IMAC, GST Tagged Purification, Talon resin, Glutathione Sepharose, Heparin Sepharose, Streptavidin Sepharose, anti-Flag, Protein A, Protein G )
» Ion Exchange Chromatography ( Mono-Q, Q Sepharose Fast Flow, Resource Q, Q Sepharose XL, ANX,  DEAE Sepharose, SP Sepharose, CM Sepharose )
» Hydrophobic Interaction Chromatography Phenyl and (CH2)n Sepharose )
» Size Exclusion Chromatography

Endotoxin Removal Service:

We manage endotoxin removal from the first purification step. Starting with exhaustively cleaned and validated equipment, we use detergent-free chromatography methods to lower endotoxin levels. 

Important Information :
 

Kindly download the information brochure to know cost of services, terms and conditions, confidentiality agreement etc.

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Enquiry at : mail@allelelifesciences.com

Call at :  +91 -  08377082003 ( Technical Coordinator )

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