Polyclonal antibodies from serum of immunized animals.
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Antibody
purification is a process used to
isolate and obtain highly purified
antibodies from a mixture of various
proteins and molecules, so you get a clean,
concentrated, functional antibody for
research, diagnostics, or therapeutic
use .
»
Protein A / Protein G / Protein L
Affinity Chromatography
»
Antigen-Specific Affinity Chromatography
»
Ammonium
Sulfate Precipitation
»
Ion Exchange Chromatography
»
Size
Exclusion Chromatography (SEC)
We are dedicated to customer service and can help you determine which purification process will provide you with the best balance among yield, purity, and cost.
Antibody
purification services can simplify the
process of extracting antibodies from
antiserum or hybridoma and cell
cultures. Immunoglobulins can be
purified via affinity binding to
immobilized protein A or protein G
particles or other means of affinity
chromatography. ion exchange or size
exclusion chromatography for further
purification, as well as endotoxin
removal for in vivo applications.
- Immuno-Affinity Purification
- IgY Purification
- Protein A purification or Protein G purification i
These services typically use affinity
chromatography techniques, such as
Protein A, G, or L columns, to
selectively bind antibodies based on
their Fc or light chain regions. For
higher specificity, antigen-specific
affinity purification can be used.
We Serve
- Research
& Development
- Health
diagnostics screening
-
Therapeutic
Development
Note: Your delivered report includes your run's actual UV trace, yield, and purity data.
A standard purification uses Affinity Chromatography for initial capture, Ion-Exchange and Hydrophobic Interaction Chromatography for intermediate impurity removal, and Size-Exclusion Chromatography for final aggregate polishing.
Antibody purification is essential to remove host
cell contaminants, proteases, and endotoxins that cause assay background,
cross-reactivity, protein degradation, and in vivo toxicity.
The sample may not bind due to excessively high salt concentration, inappropriate pH conditions and buffer components. Column Efficiency may be improved by optimizing column conditions and sample loading conditions.
