Biopharma and protein biology bridge the gap between biological science and industrial production. Whether you are aiming to produce therapeutic antibodies, vaccines, industrial enzymes and other biological products.
Unit 1 - Upstream & Downstream Processing:
Bio-Process, Growth Curve & cell count, Production of
biomass & measurement
Unit 2 - Partial Protein
Purification: Enzyme Activity Assay, Protein
Extraction, Ammonium Precipitation, Desalting and Partial
Purification
Unit 3 - Protein Purification &
Chromatography: Hands on experience with FPLC
systems, Sample Preparation,Fractionation Strategies and
Buffer preparation
Unit 4 - Hands on experience
with FPLC/AKTA systems: FPLC Sample run,Real-Time
Trace Diagnostics, System back pressure, pH, Conductivity
and data analysis
Unit 5 - Analytical
Characterization & Bioassays: Protein
Quantification and SDS-PAGE Analysis
Unit 6 -
Amino Acid Analysis by HPLC: Protein Derivatization, Preparation & Amino Acid Analysis by HPLC
| Unit | Details | Technologies / Instrumentation |
|---|---|---|
| 1 | Upstream & Downstream | Microbial Culture, bioprocess, culture optimisation |
| 2 | Partial Purification | Ammonium sulfate fractionation, Desalting and Enzymatic assay |
| 3 | FPLC Purification | Fast Protein Liquid Chromatography & Purification Strategies |
| 4 | Analytical Bioassays | SDS-PAGE & Protein Analysis by Microplate Reader |
| 5 | Amino Acid Analysis | Derivatization and Amino Acid Analysis by HPLC |
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Production of Monoclonal and Polyclonal Antibodies
- Production of Vaccine
- Production of insulin,
hormones and other therapeutic proteins
- Production of Industrial
enzymes
- Life Science
Research
Mastering hands-on protein purification, HPLC analysis, and bioassays qualifies you for high-demand Process Development, Validation, and Quality Control roles across biomanufacturing, CROs, and R & D.
FPLC and HPLC training connects biomolecule production with precision analysis. FPLC teaches scalable, preparative protein purification, while HPLC provides the high-resolution separation essential for quality control, aggregate quantification, and purity testing.
Duration: 80 Hrs or Up to 45 Days
Service Charges: We charge fee to cover the expenses of chemical and reagents that is INR 12,000 /- (Indian Nationals) & $200 (Foreign Nationals)
Note: The program utilizes advanced instrumentation, specialized chromatographic resins, and high-grade analytical reagents. Because the fee directly covers these extensive consumable costs and dedicated instrument, the fee structure is fixed and non-negotiable.
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