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The intersection of forensic DNA profiling, PCR amplification, and HPLC analysis forms the operational bedrock of modern criminal justice, molecular diagnostics, and chemical toxicology.
Unit 1 - Forensic Sample Processing & DNA Profiling:
Collection of forensic samples, DNA Extraction, Quantitative
& Qualitative Analysis of DNA.
Unit 2 -
Mitochondrial DNA & Quantitative qPCR: Mt- DNA
Extraction, Basics of Real Time PCR, Primer Design, sample
run and data analysis.
Unit 3 - Forensic
Toxicology & Matrix Clean-up: Solid Phase
Extraction of toxins, Clean-up using Alumina and Silica
Column, Spectrophotometric & Chromatography Analysis.
Unit 4 - Physio-Chemical Characterization:
Determination of moisture, Acid Value, Iodine Value and
Specific Gravity.
Unit 5 - Toxin Analysis & HPLC:
Sample Preparation, Solid-phase extraction, Derivatization
for toxins, Analysis of toxic compound by HPLC.
| Unit | Details | Technologies / Instrumentation |
|---|---|---|
| 1 | DNA Extraction & Analysis | Electrophoresis, Spectrophotometry, PCR & Gel Doc |
| 2 | Mt. DNA & Real Time PCR | Mt.DNA Extraction, Real-Time Thermal Cycler, Primer 3 & Software |
| 3 | Forensic Toxicology | SPME, Spectrophotometry, Column Purification |
| 4 | Chemical Characterization | Karl Fischer Titrator, Titration, Spectrophotometry |
| 5 | Toxin Analysis & HPLC | High Performance Liquid Chromatography |
Beyond forensics analysis, these high-precision techniques are widely applied in pharmaceutical quality control, cosmetics analysis and the regulatory monitoring of controlled substances.
Hands-on DNA extraction, quantification, and toxin screening experience directly maps to roles in forensic labs, law enforcement crime units, and toxicology testing facilities, giving you courtroom-relevant technical grounding.
Training covers DNA and mitochondrial DNA extraction from forensic samples, quantitative and qualitative DNA analysis, Real-Time PCR for identification, and solid-phase extraction methods for toxin screening.
Duration: 80 Hrs or Up to 45 Days
Service Charges: We charge fee to cover the expenses of chemical and reagents that is INR 12,000 /- (Indian Nationals) & $200 (Foreign Nationals)
Note: The program utilizes advanced instrumentation, specialized chromatographic resins, and high-grade analytical reagents. Because the fee directly covers these extensive consumable costs and dedicated instrument, the fee structure is fixed and non-negotiable.
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