As the operational underpinning for drug development, diagnostics, and clinical translation, mastery of these fundamental immunotechniques is highly valued in both academia and the biopharmaceutical sector.
Unit 1 - In-Vitro Cell Culture Fundamentals &
Laboratory Safety: Biosafety & Aseptic techniques,
decontamination methods, Media Formulations &
Additives,Sterilization & Quality Control.
Unit 2
- Primary Cell Isolation, Quantification & Inverted
Microscopy: Tissue dissociation methods, Cell
counting and viability, alignment of inverted phase-contrast
optics and morphological evaluation.
Unit 3 -
Enzyme-Linked Immunosorbent Assay (ELISA): Direct
vs. Indirect ELISA design, signal generation and
amplification, standard curve preparation and dynamic range
optimization.
Unit 4 - Blocking Chemistry,
SDS-PAGE & Western Blotting: Protein quantitation
by Bradford, sample preparation & gel electrophoresis,
membrane transfer (PVDF), blocking & immunodetection.
Unit 5 - Immunofluorescence (IFA), High-Resolution
Imaging & Quantitative Analysis: Sub-culturing onto
glass coverslips, fixation & permeabilization, fluorescent
staining & microscopy, image acquisition and analysis.
| Unit | Details | Technologies / Instrumentation |
|---|---|---|
| 1 | In-Vitro Cell Culture | Media Preparation, Biosafety, CO2 Incubator & Sterlization technique |
| 2 | Primary Cell Isolation | Extraction of tissues, viability and cell analysis |
| 3 | ELISA Assay | ELISA assay by microplate reader |
| 4 | SDS-PAGE & Western Blotting | SDS-PAGE, Western Blot, Chemiluminescence |
| 5 | Immunofluorescence (IFA) | Analysis by Fluorescence Microscopy & Data Analysis |
In research, this powerful combination is widely applied in fields like oncology, immunology, and drug discovery.
Hands-on ELISA and immunoassay experience is a core requirement for roles in diagnostics, quality control, and immunology research, helping you build a practical skillset that's directly applicable to lab and biotech industry positions.
Training includes primary cell isolation and counting under an inverted microscope, along with a full ELISA workflow using a microplate reader for signal detection and quantification.
Duration: 80 Hrs or Up to 45 Days
Service Charges: We charge fee to cover the expenses of chemical and reagents that is INR 12,000 /- (Indian Nationals) & $200 (Foreign Nationals)
Note: The program utilizes advanced instrumentation, specialized chromatographic resins, and high-grade analytical reagents. Because the fee directly covers these extensive consumable costs and dedicated instrument, the fee structure is fixed and non-negotiable.
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