Real-time PCR, often known as Q-PCR, is a technique used to quantify DNA, either genes or, more frequently, reverse-transcribed RNA to cDNA. Similar to a standard PCR reaction, the idea behind real-time PCR is that primers are used to amp up DNA, but after each amplification cycle, fluorescence in relation to the quantity of PCR product is measured. Real-time PCR, also known as quantitative PCR or qPCR, is currently a widely used technique in the clinical and scientific domains for the identification, measurement, and typing of various microbial agents. Food safety as well as veterinary diagnosis..
Molecular Genetics & Healthcare
Unit I- Nucleic Acid Extraction (DNA & RNA)
- DNA Extraction & Optimization of Protocol
- Quantitative & Qualitative Analysis of DNA
- RNA Extraction & Optimisation
- Quantitative & Qualitative Analysis of RNA
Unit II- mRNA Purification and cDNA Synthesis, Qualitative RT-PCR
- RNA Extraction and mRNA Purification
- First Standard cDNA Synthesis
- Optimisation of PCR Reaction and PCR run
- Qualitative RT- PCR sample run & Analysis
Unit III-Primer Design, Molecular Diagnostics, SNP Detection and Multiplexing
- PCR run for Molecular Diagnostics
- Analysis of food samples by PCR Multiplexing
- SNP (Single Nucleotide Polymorphism) Detection
- PCR for DNA Barcoding Analysis
Unit IV-Real Time PCR Assay & Basics of Microarray Technology
- RNA Extraction and First Standard cDNA Synthesis
- Primer Design for Real Time PCR, Basics of Q-PCR
- Real Time PCR run with SYBR Green Assay
- Real Time PCR Data Analysis & Basics of microarray
Frequently Asked Questions
Dedicated Lab Space:- Modern biology and medicine are based on molecular genetics. It allows us to decipher the genetic data that is encoded in our RNA and DNA.
PCR Hood: In genetic and molecular biology labs, PCR hoods are used to stop sample contamination.
Thermocycler: PCR has many research and practical applications. It is routinely used in gene expression,DNA cloning, medical diagnostics, and forensic analysis.
Real Time PCR: The primary characteristic of RT-PCR is the "real time" detection of DNA amplification while the PCR is running. Using a "fluorescent reporter," which monitors the fluorescent signal to determine the amount of amplified DNA molecules directly, the PCR progression is evaluated.
Microarray & Sequencer: Thousands of DNA sequences can be analyzed simultaneously using microarrays for genetic research and diagnostic purposes.
DNA Concentrator: The DNA Concentrator is used to efficiently concentrate DNA/RNA from various samples.
Other Basic Lab Equipments: Other equipments used in molecular biology labs are distilled water unit,ICE flaking machine,electrophoresis units, Gel documentation system with gel analysis software, linear current power supply, refrigerated centrifuges, water bath,dry bath etc.
Bioinformatics: There are several great online tools and platforms for learning bioinformatics, depending on your level and interests like; IIT virtual Learning Platform,NCBI Bioinformatics Resources.
Basic Lab Techniques: Basic calculation of chemical preparation (use graph pad or molarity dilution app), buffer preparation, Pipetting,pH, sterlization etc is included in every course..
Note: All basic experiments of qualitative and quantitative analysis of various types of products, basic microbiology, basic in vitro cell culture, and PCR-based experiments will be available on our YouTube channel from time to time.
Our Pedagogy evolve to make education more experiential, inquiry-driven, discovery-oriented, learner-centred, discussion-based, flexible, and enjoyable.
Validated Lab Manual provides a platform for understanding the basic of experiments
Be a volunteer oppurtunity- Assisting fellow lab students will boost your self-esteem and cultivate your leadership skills.
Our Guidance- Lab scientists and technicians will have one-on-one interactions with students and help them develop their own lab abilities.
Eligibility: Students from life science, biotechnology, chmeistry, forensic science,food science and pharmaceutical science may apply.
Fee: INR 12,000/- For Indian Citizens.
Duration: 85 Hours or Upto One Month (maximum)
State of art lab facility: We provide the best lab space for working in chemistry and biology. Students use cutting-edge tools firsthand while being supervised by knowledgeable team members.CHECK LAB FACILITY
Bridging the Gap Between Theory and Practice:
Immersive Learning: Students who immerse themselves in practical activities get essential insights and experience that help them prepare for issues they may face in their future employment.
Application of In-vitro Cell Culture: Students who immerse themselves in practical activities get essential insights and experience that help them prepare for issues they may face in their future employment.
How to select training place andtraining program? You are strictly advice to discuss the Full Summer Training Brochure with your Faculty of your institution or Scientist in your relation.Their advice will be crucial for your career.
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